狼山鸡NLRC5基因启动子区SNP及其在先天免疫中的效应分析

doi:10.11843/j.issn.0366-6964.2016.01.006

Abstract

Abstract:

This study was conducted to detect single nucleotide polymorphisms（SNPs）in NLRC5 gene promoter region of Langshan chicken，and further determine genetic effects of different genotypes which were verified by progeny segregating group，aiming to develop genetic markers associated with innate immune traits.MALDI-TOF-MS method was applied to scan SNPs in NLRC5 gene promoter region；afterwards，some immune traits and their differences between individuals with different genotypes were analyzed.The SNP scanning results found A/G mutation at g.628981 site，along with 3 genotypes，namely，AA，AG and GG.The immune indexes presented significant difference among individuals with different genotypes.The H/L value of individuals with AA genotype was significantly lower than that of individuals with GG genotype，but spleen index，thymus index，lymphocyte transformation rate and content of IgG were significantly higher than that of individuals with GG genotype (P<0.05)；it was also found that the traits of individuals with AA genotype were better than that of individuals with GG genotype in F₂ generation of AA and GG segregating groups.Collectively，combining with the transcription differences of NLRC5 gene in thymus and spleen tissues，our study indicate that birds with AA genotype show better performances of innate immunity and g.628981(A/G) site of NLRC5 gene can be used as an effective genetic marker of innate immune traits.

CLC Number:

S813.2

LIU Xiang-ping，GUO Xiao-min，LI Zhi-teng，ZHU Jing，SHENG Zhong-wei，MA Teng，CHANG Guo-bin，DOU Xin-hong，WANG Ke-hua，CHEN Guo-hong. SNPs of Promoter Region of NLRC5 Gene and Its Genetic Effect on Innate Immune Traits in Langshan Chicken Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 41-47.

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SNPs of Promoter Region of NLRC5 Gene and Its Genetic Effect on Innate Immune Traits in Langshan Chicken Breeds

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